Chromatin targeting of nuclear pore proteins induces chromatin decondensation

Ectopically tethered CP190 induces large-scale chromatin decondensation

Insulator mediated alteration in higher-order chromatin and/or nucleosome organization is an important aspect of epigenetic gene regulation. Recent studies have suggested a key role for CP190 in such processes. In this study, we analysed the effects of ectopically tethered insulator factors on chromatin structure and found that CP190 induces large-scale decondensation when targeted to a condens...

The nuclear pore complex and chromatin boundaries.

The nuclear pore as a chromatin boundary

he Cajal body (CB, formerly coiled body) is a prominent nuclear structure, yet its function remains obscure. Now, speculate that the CB is where small nuclear RNAs go for modification. Last year, Kiss's team identified a small guide RNA, U85, that directs both 2'-O-methylation and pseudouridylation of the spliceosomal component U5 snRNA. This year, they've identified six more RNAs, Novel guide ...

the effect of glutathione on nuclear chromatin decondensation of human sperm

introduction: during spermiogendsis, sperm chromatin histones are replaced by protamine. after fertilization, the sprem nucleus is decondenced by glutathione (gsh). in this survey, we study the effect of various concentrations of gsh on human in vitro sperm decondensation. material and methods: after preparation and analysis of semen samples, sperm pellet was washed by ham's f-10 medium twice a...

Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin

Nuclear pore complexes (NPCs) form the gateway to the nucleus, mediating virtually all nucleocytoplasmic trafficking. Assembly of a nuclear pore complex requires the organization of many soluble sub-complexes into a final massive structure embedded in the nuclear envelope. By use of a LacI/LacO reporter system, we were able to assess nucleoporin (Nup) interactions, show that they occur with a h...